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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
18/04/2024 |
Data da última atualização: |
18/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, E. R. dos; CAMARGO, B. R. de; SILVA, L. A. da; LAUMANN, R. A.; RIBEIRO, B. M.; ARDISSON-ARAÚJO, D. M. P. |
Afiliação: |
ETHIANE ROZO DOS SANTOS, UNIVERSIDADE DE BRASÍLIA; BRENDA RABELO DE CAMARGO, UNIVERSIDADE DE BRASÍLIA; LEONARDO ASSIS DA SILVA, UNIVERSIDADE DE BRASÍLIA; RAUL ALBERTO LAUMANN, CENARGEN; BERGMANN MORAIS RIBEIRO, UNIVERSIDADE DE BRASÍLIA; DANIEL M. P. ARDISSON-ARAÚJO, UNIVERSIDADE DE BRASÍLIA. |
Título: |
The multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera: Platygastridae). |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, 2024. |
DOI: |
https://doi.org/10.1007/s42770-024-01340-y |
Idioma: |
Português |
Notas: |
On-line first. |
Conteúdo: |
Wasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector. MenosWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological si... Mostrar Tudo |
Palavras-Chave: |
Egg parasitoid; Halyomorpha halys virus; Stinkbug; Telenomus podisi. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02558naa a2200253 a 4500 001 2163741 005 2024-04-18 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s42770-024-01340-y$2DOI 100 1 $aSANTOS, E. R. dos 245 $aThe multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera$bPlatygastridae).$h[electronic resource] 260 $c2024 500 $aOn-line first. 520 $aWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector. 653 $aEgg parasitoid 653 $aHalyomorpha halys virus 653 $aStinkbug 653 $aTelenomus podisi 700 1 $aCAMARGO, B. R. de 700 1 $aSILVA, L. A. da 700 1 $aLAUMANN, R. A. 700 1 $aRIBEIRO, B. M. 700 1 $aARDISSON-ARAÚJO, D. M. P. 773 $tBrazilian Journal of Microbiology, 2024.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
11/12/2007 |
Data da última atualização: |
08/06/2017 |
Autoria: |
BASTOS, C. S.; SUINAGA, F. A.; RANGEL, L. E. P.; CHITARRA, L. G.; MENEZES, V. L.; LIRA, A. J. S.; CABRAL, M. C. C.; ARAÚJO, D. E. B. |
Afiliação: |
Embrapa Algodão. |
Título: |
Inimigos naturais de artrópodes associados à cultura do algodão |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Campina Grande: Embrapa Algodão, 2002. |
Páginas: |
não paginado |
Idioma: |
Português |
Notas: |
Folder |
Palavras-Chave: |
Artrópodes; Inimigos naturais; Pragas do algodão. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/38886/1/Folder-inimigos-naturais-2002.pdf
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Marc: |
LEADER 00665nam a2200241 a 4500 001 1276174 005 2017-06-08 008 2002 bl uuuu u0uu1 u #d 100 1 $aBASTOS, C. S. 245 $aInimigos naturais de artrópodes associados à cultura do algodão 260 $aCampina Grande: Embrapa Algodão$c2002 300 $anão paginado 500 $aFolder 653 $aArtrópodes 653 $aInimigos naturais 653 $aPragas do algodão 700 1 $aSUINAGA, F. A. 700 1 $aRANGEL, L. E. P. 700 1 $aCHITARRA, L. G. 700 1 $aMENEZES, V. L. 700 1 $aLIRA, A. J. S. 700 1 $aCABRAL, M. C. C. 700 1 $aARAÚJO, D. E. B.
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